Recycling composted human feces as biofertilizer for crop production: Assessment of soil and lettuce plant tissue contamination by Escherichia coli and human adenovirus.

Using waste from sewage systems, particularly human excreta, could save resources and increase soil fertility, contributing to nutrient management. However, because of the pathogenic content in human feces, this resource can pose health risks to farmers and consumers. Therefore, this work analyzed the behavior of the microorganisms: Escherichia coli ATCC13706 and human adenovirus (HAdV-2) in the soil and the internal part of the plant tissue during the vegetative stage after applying spiked composted human feces as biofertilizer. In a greenhouse, we simulated the application of the biofertilizer in lettuce cultivation by spiking three concentrations of E. coli (6.58, 7.31, and 8.01 log10 CFU.g-1) and HAdV-2 (3.81, 3.97, and 5.92 log10 PFU.g-1). As a result, we achieved faster decay in soil at higher concentrations of E. coli. We estimated linear decay rates of -0.07279, -0.09092, and -0.115 days, corresponding to T90s of 13.7, 11.0, and 8.6 days from higher to smaller concentrations of E. coli, respectively. The estimated periods for the inactivation of 4 logarithmic units of E. coli bacteria in soil are longer than the cultivation period of lettuce for all concentrations studied. Concerning the bacterial contamination in plants, we found E. coli in the internal part of the leaves at the highest concentration tested during the first three weeks of the experiment. Furthermore, HAdV-2 was found in roots at a stable concentration of 2-2.3 log10 PFU.g-1 in five of the six samples analyzed. Therefore, bacterial infection could pose a risk, even if fresh greens are washed before consumption, especially for short-term cultures. Regarding viral infection, a positive result in the roots after disinfection may pose a risk to root and tubercule vegetables. These discoveries highlight the importance of conducting comprehensive evaluations of hygiene practices in incorporating organic amendments in crops, explicitly aiming to minimize the risk of post-contamination.

The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019.

Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L-1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L-1, ranging from 5.49 ± 0.02 log10 GC L-1 (27th November 2019) to 6.68 ± 0.02 log10 GC L-1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020).

Attitudes of food consumers at universities towards recycling human urine as crop fertiliser: A multinational survey dataset.

We present here a data set generated from a multinational survey on opinions of university community members on the prospect of consuming food grown with human urine as fertiliser and about their urine recycling perceptions in general. The data set comprises answers from 3,763 university community members (students, faculty/researchers, and staff) from 20 universities in 16 countries and includes demographic variables (age bracket, gender, type of settlement of origin, academic discipline, and role in the university). Questions were designed based on Ajzen's theory of planned behaviour to elicit information about three components of behavioural intention-attitudes, subjective norms, and perceived behavioural control. Survey questions covered perceived risks and benefits (attitudes), perceptions of colleagues (injunctive social norm) and willingness to consume food grown with cow urine/faeces (descriptive social norm), and willingness to pay a price premium for food grown with human urine as fertiliser (perceived behavioural control). We also included a question about acceptable urine recycling and disposal options and assessed general environmental outlook via the 15-item revised New Ecological Paradigm (NEP) scale. Data were collected through a standardised survey instrument translated into the relevant languages and then administered via an online form. Invitations to the survey were sent by email to university mailing lists or to a systematic sample of the university directory. Only a few studies on attitudes towards using human urine as fertiliser have been conducted previously. The data described here, which we analysed in "Willingness among food consumers at universities to recycle human urine as crop fertiliser: Evidence from a multinational survey" [1], may be used to further understand potential barriers to acceptance of new sanitation systems based on wastewater source separation and urine recycling and can help inform the design of future sociological studies.

Willingness among food consumers to recycle human urine as crop fertiliser: Evidence from a multinational survey.

Source-separating sanitation systems offer the possibility of recycling nutrients present in wastewater as crop fertilisers. Thereby, they can reduce agriculture's impacts on global sources, sinks, and cycles for nitrogen and phosphorous, as well as their associated environmental costs. However, it has been broadly assumed that people would be reluctant to perform the new sanitation behaviours that are necessary for implementing such systems in practice. Yet, few studies have tried to systematically gather evidence in support of this assumption. To address this gap, we surveyed 3763 people at 20 universities in 16 countries using a standardised questionnaire. We identified and systematically assessed cross-cultural and country-level explanatory factors that were strongly associated with people's willingness to consume food grown using human urine as fertiliser. Overall, 68% of the respondents favoured recycling human urine, 59% stated a willingness to eat urine-fertilised food, and only 11% believed that urine posed health risks that could not be mitigated by treatment. Most people did not expect to pay less for urine-fertilised food, but only 15% were willing to pay a price premium. Consumer perceptions were found to differ greatly by country and the strongest predictive factors for acceptance overall were cognitive factors (perceptions of risks and benefits) and social norms. Increasing awareness and building trust among consumers about the effectiveness of new sanitation systems via cognitive and normative messaging can help increase acceptance. Based on our findings, we believe that in many countries, acceptance by food consumers will not be the major social barrier to closing the loop on human urine. That a potential market exists for urine-fertilised food, however, needs to be communicated to other stakeholders in the sanitation service chain.

Sanitary effectiveness and biogas yield by anaerobic co-digestion of swine carcasses and manure.

The present study evaluated anaerobic co-digestion of swine manure and swine carcasses for biogas yield and inactivation/behaviour of pathogens purpose. Biochemical Methane Production tests were performed with samples containing ratios of 3, 7.5 and 15 kgcarcass m-3 manure. For pathogens inactivation experiments known amounts of model microrganisms (sensitive and resistant) were artificially inoculated in anaerobic reactors at 24°C and 37°C. The addition of carcass resulted in an increase until 119% of biogas yield compared to swine manure mono-digestion. Salmonella enterica, Escherichia coli and PCV2 were reduced >3log10 (24°C or 37°C) during 30 days. At 37°C, MS2 and PhiX-174 were reduced 3log10 and 1.8log10, respectively. At 24°C, MS2 reduced 1.5 log10 and PhiX-174 did not present any decay over 30 days. Considering the most resistant biomarkers pathogens, as bacteriophage, we recommend the swine carcasses pre-treatment, such as high temperatures, for sanitary security.

Higienização de lodo de esgoto em reator com aquecimento solar: inativação de coliformes totais e Escherichia coli / Sanitisation of sewage sludge in a solar heated reactor: inactivation of total coliforms and Escherichia coli

RESUMO O uso de lodo de esgoto na agricultura se tornou prática corrente em diversos países, sendo atrativa em muitos aspectos, principalmente no fornecimento de matéria orgânica e nutrientes ao solo. Embora os benefícios ambientais e agrícolas do uso de lodo sejam consideráveis, tal prática deve ser realizada de forma sanitariamente segura. Nesse contexto, o objetivo deste estudo foi avaliar a eficiência de um processo de higienização térmica de lodo de esgoto utilizando energia solar. O lodo era aquecido através de um trocador de calor, construído com tubos de cobre e instalado dentro do reator, no qual circula água aquecida em coletores solares planos. Foram realizados 16 ensaios experimentais em diferentes condições de irradiação solar. A inativação térmica da Escherichia coli foi avaliada através do modelo cinético de primeira ordem em condições não isotérmicas. O processo mostrou-se eficiente em ensaios realizados com irradiação solar média do período acima de 500 W.h.m-2, com redução de E. coli entre 4,2 e 7,1 log10 e de coliformes totais entre 4,8 e 7,4 log10. Os ensaios realizados em dias com menores índices de irradiação solar tiveram a eficiência de higienização comprometida, devido às baixas temperaturas atingidas pelo lodo.

ABSTRACT The application of sewage sludge in agriculture has become a common practice in many countries, which is attractive in many aspects, especially for the input of organic matter and nutrients in the soil. Despite the benefits of sludge use in agriculture and for the environment , this practice needs to be conducted considering safety aspects regarding sanitary conditions. In this context, the aim of this study was to evaluate the efficiency of a thermal disinfection process of sewage sludge using solar energy. The sludge was heated through a heat exchanger built with copper pipes and installed inside the reactor, in which water heated in flat plate solar collectors circulates. Sixteen experimental tests were performed under different solar irradiation conditions. The thermal inactivation of Escherichia coli in the reactor was evaluated using the first order kinetic model in non-isothermal conditions. The process proved effective in those tests with an average solar irradiation period above 500 W.h.m-2, and the reduction of E. coli was between 4.2 and 7.1 units log10; and between 4.8 and 7.4 units log10 of total coliforms. When tests were conducted in days with lower levels of solar irradiation, the efficiency of sanitisation was compromised, due to the low temperatures reached by the sludge.

Inactivation of adenovirus, reovirus and bacteriophages in fecal sludge by pH and ammonia.

The aim of this study was to evaluate the inactivation of adenovirus, reovirus and bacteriophages (MS2, ΦX174, 28B) in a fecal sludge. We conducted two experiments. In the first, we tested different compositions of the fecal sludge by mixing different amounts of water, feces and urine, totaling nine combinations which were kept at temperatures between 10 and 28°C. In the second study, urea was added to the mixtures, which were kept at temperatures from 5 to 33°C. The inactivation was based on a combination of temperature, pH and uncharged ammonia concentration. The increase in pH and ammonia was provided mainly by urine content (Experiment 1) and by urine and added urea (Experiment 2). The inactivation of bacteriophages was slower than the AdV and ReV. At 23°C and 28°, reasonable treatment times were obtained when pH was higher than 8.9 and NH3 concentrations were higher than 35 and 55 mM respectively. With those conditions, the maximum time for a 3 log reduction in viruses, according to this study, would be 35 days (23°C) and 21 days (28°C). However, in most applications where helminth eggs are present, the treatment time and NH3 for sanitization will be the scaling criteria, as they are more persistent. Concerning the sanitization of effluents from latrines, vacuum toilets or dry toilets in developing countries with tropical and sub-tropical climates, the use of intrinsic ammonia combined with high pH can be effective in producing a safe and highly valuable liquid that can be used as a fertilizer. In the case of the fecal sludge with very intrinsic ammonia concentration (<20 mM), sanitization could still be achieved by the addition of urea.

Experiências brasileiras com wetlands construídos aplicados ao tratamento de águas residuárias: parâmetros de projeto para sistemas horizontais / Brazilian experiences with constructed wetlands applied to wastewater treatment: design parameters for horizontal systems

A utilização no Brasil de sistemas tipo wetlands construídos no tratamento de águas residuárias iniciou no ano de 1980, intensificando-se a partir do ano 2000. O objetivo deste trabalho é apresentar um estado da arte da aplicação no Brasil dos wetlands construídos de escoamento subsuperficial de fluxo horizontal (WCFH), identificando parâmetros de projeto e dados operacionais. Aborda-se, inicialmente, uma classificação para os wetlands construídos, seguido dos parâmetros de projeto e operação dos WCFH, destacando os diferentes critérios de dimensionamento, tais como os modelos oriundos da cinética de primeira ordem aplicável aos reatores tipo pistão, a relação de área per capita, o carregamento orgânico superficial e a taxa hidráulica, bem como a composição das unidades de tratamento e as macrófitas empregadas.

The implementation of constructed wetlands for wastewater treatment in Brazil has started in the eighties, being intensified since the year 2000. The aim of this study is to present the state of art of implementation of horizontal sub-superficial flow constructed wetlands (HFCW) in Brazil, focusing on the identification of design parameters and operational data. Initially a classification for constructed wetlands is given, followed by design parameters and operational conditions for HFCW, highlighting different project criteria, e.g. models from first order kinetics for tubular piston reactors, the relation of superficial area per person, the organic superficial loading and the hydraulic loading, as well as the composition of units and macrophytes utilised.

The potential for self-sanitisation of faecal sludge by intrinsic ammonia.

Faecal sludge has the potential to be used as a sustainable fertiliser in agriculture, but the sludge must be sanitised due to its content of pathogenic microorganisms. The intrinsic ammonia from the urine may be sufficient for sanitisation of the sludge if it is not too diluted by flush water or lost by ventilation. To evaluate the potential for this sanitisation method, inactivation of Enterococcus faecalis, Salmonella typhimurium and Ascaris suum eggs during treatment were assessed. The inactivation was studied at different storage temperatures (10-28 °C) and in several sludge mixes with different contents of urine, faeces and flush water, and with ammonia concentrations from 40 to 400 mM. All pathogens were inactivated by the ammonia, and ascaris eggs were the most persistent. Lower flush water volume and higher urine content favoured inactivation, mainly due to increased uncharged ammonia (NH3) concentration. The lag phase in ascaris inactivation was shortened by increasing temperature and NH3 concentration, while post-lag phase inactivation was not influenced by NH3 concentration. Faecal sludge can be sanitised by airtight storage without the use of additives when flush water volumes are sufficiently low. For temperatures of 23-28 °C, a 3 log reduction of ascaris egg viability can be achieved within 1-6 months depending on ammonia concentration and temperature.

Otimização do processo de nitrificação com o uso de conchas de ostras como material suporte em reatores aeróbios com biomassa fixa / Optimization of the nitrification process using oyster shells as a support material in fixed biomass aerobic reactors

A preservação dos mananciais de água traz como ponto de atuação primordial o tratamento dos esgotos sanitários a nível terciário. A partir daí destaca-se a necessidade de otimizar os sistemas de remoção de nutrientes, buscando-se processos mais estáveis, economicamente viáveis e ambientalmente sustentáveis. O objetivo desta pesquisa foi avaliar conchas de ostras como material de preenchimento em um biofiltro aerado submerso, assumindo-se a hipótese de que as mesmas podem interagir com a fase líquida do reator, fornecendo alcalinidade necessária à estabilidade do processo de nitrificação autotrófica. Uma série de condições operacionais foi avaliada visando à nitrificação; portanto, foi possível concluir que, no biofiltro aerado submerso com conchas de ostras, podem ser aplicadas taxas hidráulicas >5,2 m³.m-2.d-1 e tempo de detenção hidráulico <7,5 horas, desde que a relação carbono: nitrogênio seja mantida abaixo de quatro. As conchas de ostras mantiveram o sistema tamponado (pH=7,5±0,3), mesmo com uma intensa atividade nitrificante no reator (100%).

One of the main key actions for water resources preservation is to promote wastewater treatment in a tertiary level. From this point, it can be highlighted the demands for optimizing the processes for nutrients removal aiming more stable processes, which are economically viable and environmentally sustainable. The aim of this research was to evaluate oyster shells as support material in an aerated submerged biofilter, having as main hypothesis the fact that they can interact with the liquid phase of the reactor, providing the necessary alkalinity to stabilize the autotrophic nitrification. The reactor studied was submitted to several operational conditions and could be concluded that, in the aerated submerged biofilter with oyster shells, hydraulic loadings can be >5.2 m³.m-2.d-1 and a hydraulic retention time <7.5 hours can be applied, since that the relation carbon: nitrogen is maintained under four. The oyster shells kept the system buffered (pH=7.5±0.3), even with a high nitrifying activity in the reactor (100%).

Faecal sludge management with the larvae of the black soldier fly (Hermetia illucens)--from a hygiene aspect.

Inadequate and lacking sanitation and wastewater treatment systems can lead to the spreading of diarrhoeal diseases. One contributing factor in the lack of such treatment systems is the lack of economic incentives for stakeholders throughout the service chain. However, the organic fraction of the waste is high in valuable plant nutrients and could be reused in agriculture and as animal feed. For example, grown larvae of the black soldier fly, Hermetia illucens L. (Diptera: Stratiomyidae), make an excellent protein source in animal feed, while the feeding activity of the larvae substantially reduces the dry mass of the treated material. This study examined the effect of black soldier fly larvae on the concentration of pathogenic microorganisms in human faeces and found a 6 log10 reduction in Salmonella spp. in human faeces in eight days, compared with a <2 log10 reduction in the control. No increased reduction was observed for Enterococcus spp., bacteriophage ΦX174 or Ascaris suum ova.

Inactivation of pathogens in feces by desiccation and urea treatment for application in urine-diverting dry toilets.

Ecological sanitation technologies can be effective in providing health and environmental pollution control if they can efficiently reduce the pathogenicity of microorganisms carried in fecal material to safe levels. This study evaluated the sanitizing effects of different additives for dry treatment of feces from urine-diverting dry toilets, based on inactivation of Enterococcus faecalis, Salmonella enterica serovar Typhimurium, bacteriophages MS2 and ΦX, and Ascaris suum. The additives, ash (A) and oyster shell (O) in different amounts and urea (U) to optimize the process, were compared with no additive, solely urea, and sawdust as controls (C) and were covered ([x%O:A]) or uncovered (x%O:A). The main inactivation factors found were desiccation, ammonia content, and pH. S. Typhimurium and E. faecalis were more affected by the ammonia content. A combination of neutral to high pH and desiccation was most effective for inactivation of MS2, and desiccation was most effective for inactivation of ΦX and A. suum. The inactivation rate was modeled for all combinations studied. The most promising treatments were [150%O:A+U], 150%O:A+U, and 150%O:A. According to the models, these could inactivate, for example, 7 log(10) units of all bacteria and bacteriophages within 83, 125, and 183 days, respectively. The inactivation of A. suum was modeled, albeit the measured decay in egg viability was low.

Filtros plantados com macrófitas de fluxo vertical aplicados na mineralização e desaguamento de lodo de tanque séptico / Vertical flow constructed wetlands for septic sludge mineralization and dewatering

O lodo acumulado no interior de tanques sépticos deve passar por um tratamento antes de sua adequada disposição final, pois apresenta uma fração da matéria orgânica não estabilizada, elevado teor de umidade e organismos patogênicos. Como alternativa para o tratamento do lodo, os filtros plantados com macrófitas são uma tecnologia promissora. Para se avaliar o potencial dos filtros no desaguamento e mineralização do lodo de tanque séptico, foram construídos dois filtros pilotos (F1 e F2) aplicando-se duas taxas de sólidos totais (ST) - F1: 250 kgST.m-2.ano-1; F2: 125 kgST.m-2.ano-1. Os resultados revelaram que o F2 apresentou melhor desempenho em relação ao F1 em termos de remoção de sólidos totais (96 por cento), demanda química de oxigênio (99 por cento) e nitrogênio amoniacal (72 por cento). O F2 proporcionou melhor desaguamento, com 67 por cento de umidade no lodo acumulado no leito e 33 por cento de sólidos totais.

The accumulated sludge in septic tanks has to be treated before the adequate final disposal, because of its high concentration of non-established organic matter, elevated content of moisture and pathogenic organisms. As an alternative to the treatment of sludge, the vertical flow constructed wetlands are a promising technology. To evaluate the potential of the filters in dewatering and mineralization of septic tank sludge, two pilot-scale constructed wetlands (CW1 and CW2) were implanted according with two total solids loads (TS) - CW1: 250 kgTS.m-2.year-1; CW2: 125kgTS.m-2.year-1. The results demonstrated that the CW2 had better efficiencies compared with CW1, in terms of total solids (96 percent), chemical oxygen demand (99 percent) and ammonia (72 percent) removal. CW2 also provided a better dewatering, with moisture of 67 percent in accumulated sludge and 33 percent of total solids.